packaging plasmid r8 74  (Addgene inc)

Journal: iScience

Article Title: Enzymatic activity of glycosyltransferase GLT8D1 promotes human glioblastoma cell migration

doi: 10.1016/j.isci.2022.103842

Figure Lengend Snippet:

Article Snippet: In brief, Hek293T cells were co-transfected with pGIPZ or pCDH transfer plasmids together with the viral core packaging construct pCMVdeltaR8.74 (gifted from Didier Trono; Addgene plasmid #12263) and the VSV-G envelope protein vector pMD.G.2 (gifted from Didier Trono; Addgene plasmid #12259) with the help of X-tremeGENE HP DNA transfection reagent (Sigma Aldrich).

Techniques: Proximity Ligation Assay, Mutagenesis, Construct, Recombinant, shRNA, Plasmid Preparation, Software